Exploration of Adhesion Molecule Expression in Cardiac Muscle of Early Atherosclerosis Dyslipidemic Sprague Dawley Rats

Titin A. Wihastuti1, Fitria N. Aini1, Nurul C. Lutfiana1, Teuku Heriansyah2, *, Nafisatuz Zamrudah1
1 Departement of Biomedicine, Faculty of Medicine, Brawijaya University, Malang, Indonesia
2 Departement of Cardiology, Faculty of Medicine, Syiah Kuala University, Aceh, Indonesia

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© 2018 Wihastuti et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4. 0 International Public License (CC-BY 4. 0), a copy of which is available at: https://creativecommons. org/licenses/by/4. 0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this authors at the Department of Cardiology, Faculty of Medicine, Syiah Kuala University, Aceh, Indonesia; Tel: +62 81805101827; E-mail:



This study is aimed to examine the expression of ICAM-1 and VCAM-1 in cardiac tissue of dyslipidemic Sprague Dawley rats.


Eight Sprague Dawley strain rats, with 150-200 gram body weight, were divided into two groups. The control group was fed a standard diet, the positive control group was fed a high-fat diet as our previous study for 8 weeks. The pattern of distribution of ICAM-1 and VCAM-1 in cardiac muscle cell was examined by immunofluorescence and observed with a confocal laser scanning microscope. Lipid profile was also examined at the end of the study.


Independent t-test showed no differences in ICAM-1 and VCAM-1 expression in cardiac muscle of hypercholesterol-diet-fed Sprague Dawley rat compared to control.


The expression of ICAM-1 and VCAM-1 in cardiac muscle did not change after the onset of atherosclerosis.

Keyword: ICAM-1, VCAM-1, Dyslipidemia, Molecule expression, Adhesion, Atherosclerosis, Immunofluorescence.