RESEARCH ARTICLE


Hemodynamic Effects of the Light Stabilizer Tinuvin 770 in Dogs In Vivo



Miklos Krepuska1, *, #, Marta Hubay2, #, Endre Zima3, Aniko Kovacs4, Violetta Kekesi3, Huba Kalasz5, Brigitta Szilagyi6, Bela Merkely3, Peter Sotonyi1, *
1 Heart and Vascular Center, Department of Vascular Surgery, Semmelweis University, Budapest, Hungary
2 Department of Forensic Pathology, Semmelweis University, Budapest, Hungary
3 Heart and Vascular Center, Department of Cardiology, Semmelweis University, Budapest, Hungary
4 Hungarian Institute for Forensic Sciences, Budapest, Hungary
5 Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, Hungary
6 Department of Mathematical Geometry, Institute of Mathematics, University of Technology and Economics, Budapest, Hungary


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© 2018 Krepuska et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4. 0 International Public License (CC-BY 4. 0), a copy of which is available at: https://creativecommons. org/licenses/by/4. 0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this authors at the Department of Vascular Surgery, Heart and Vascular Center, Semmelweis University, Budapest, H-1122 Budapest, Városmajor utca 68; Hungary, Tel: +(36) -1-4586734; Fax: +(36) -1-458-6746; E-mails: sotonyi@hotmail.com, krepuskam@hotmail.com# These authors contributed equally to this work


Abstract

Introduction:

Tinuvin 770 [bis(2,2,6,6-tetramethyl-4-piperidinyl) sebacate, Ciba-Geigy, Basel, Switzerland] is a UV light stabilizer that is a component of many plastic materials used world-wide in the medical and food industries. We report on the acute hemodynamic effects of Tinuvin 770 examined in dogs.

Materials and Methods:

Tinuvin 770 was dissolved in a mixture of saline and ethanol (1:1 v/v) and was administered to 12 intravenously narcotized and respirated dogs in increasing doses (T1-T7: 1, 3.3, 6.6, 10, 33.3, 66.6 and 100 mg, respectively). The doses were given as bolus injections over a three minute period, and the effects were recorded for 12 minutes. The vehicle was used as a control. Hemodynamic parameters (heart rate, blood pressure, end-diastolic pressure, dp/dt, cardiac output) and ECG were monitored continously.

Results:

At doses T1-T4, systolic and diastolic blood pressures, mean pressure and ventricular contractility were significantly decreased without significant changes in cardiac output, heart rate, or PQ interval. At doses T5 and T6, declines in blood pressure and myocardial contractility were observed. At doses T6 and T7, heart rate and PQ interval decreased substantially. Irreversible circulatory failure occured in one dog after administering dose T6 and in 8 dogs following dose T7.

Conclusion:

Tinuvin 770 induces acute hemodynamic alterations. In lower doses, it causes peripheral vasodilatation, however at higher doses acute cardiac failure occured. Plastics containing Tinuvin 770 should be used with care in medical practice and the laboratory.

Keywords: Tinuvin 770, L-type Ca2+ channel, Nicotinic acetylcholine receptor, Cardiotoxicity, Circulation, Hemodynamic parameters, dp/dt.